PrimePCR™ Probe Assay: RPL6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RPL6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RPL6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041374
Assay Design:   exonic
Chromosome Location:   12:112846359-112846452question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000440804 ENST00000250360 ENST00000202773 ENST00000424576 ENST00000549923 ENST00000549847 ENST00000553213 ENST00000551291 ENST00000548343 ENST00000549006 ENST00000416097 ENST00000548019 ENST00000348165 ENST00000383196 ENST00000552144 ENST00000310430 ENST00000518281 ENST00000349314 ENST00000361710 ENST00000397287 ENST00000397293 ENST00000424878 ENST00000379446 ENST00000504387 ENST00000338432 ENST00000377848 ENST00000377854 ENST00000568908 ENST00000568909 ENST00000567835 ENST00000562631 ENST

Gene Information

Ribosomes the organelles that catalyze protein synthesis consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L6E family of ribosomal proteins. It is located in the cytoplasm. The protein can bind specifically to domain C of the tax-responsive enhancer element of human T-cell leukemia virus type 1 and it has been suggested that the protein may participate in tax-mediated transactivation of transcription. As is typical for genes encoding ribosomal proteins there are multiple processed pseudogenes of this gene dispersed through the genome. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   RPL6
Gene Name:   ribosomal protein L6
Aliases:   L6, SHUJUN-2, TAXREB107, TXREB1
RefSeq:   NC_000012.11 NT_009775.17
Ensembl:   ENSG00000089009
Entrez:   6128
Chromosome Mapping:   12q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.040000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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