PrimePCR™ Probe Assay: BAK1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041364
Assay Design:   exonic
Chromosome Location:   6:33541477-33541681question
Amplicon Length:   175
Splice Variants Targeted:   ENST00000290378 ENST00000374467 ENST00000360661 ENST00000442998

Gene Information

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq Jul 2008]

Gene Symbol:   BAK1
Gene Name:   BCL2-antagonist/killer 1
Aliases:   BAK, BAK-LIKE, BCL2L7, CDN1, MGC117255, MGC3887
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000030110
Entrez:   578
UniGene:   Hs.485139
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 35.290000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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