PrimePCR™ Probe Assay: CDK11A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CDK11A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CDK11A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041356
Assay Design:   exonic
Chromosome Location:   1:1640305-1640486question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000513088 ENST00000407249 ENST00000317673 ENST00000340677 ENST00000341832 ENST00000361417 ENST00000356063 ENST00000356200 ENST00000378638 ENST00000404249 ENST00000357760 ENST00000358779 ENST00000378633 ENST00000378635 ENST00000405240 ENST00000357376 ENST00000337526 ENST00000394611 ENST00000404909 ENST00000354474 ENST00000344191 ENST00000351017 ENST00000392108 ENST00000447894 ENST00000400822 ENST00000342756 ENST00000601898 ENST00000600882

Gene Information

This gene encodes a member of the p34Cdc2 protein kinase family. p34Cdc2 kinase family members are known to be essential for eukaryotic cell cycle control. This gene is in close proximity to CDC2L1 a nearly identical gene in the same chromosomal region. The gene loci including this gene CDC2L1 as well as metalloprotease MMP21/22 consist of two identical tandemly linked genomic regions which are thought to be a part of the larger region that has been duplicated. This gene and CDC2L1 were shown to be deleted or altered frequently in neuroblastoma with amplified MYCN genes. The protein kinase encoded by this gene could be cleaved by caspases and was demonstrated to play roles in cell apoptosis. Many transcript variants encoding several different isoforms have been found for this gene but the full-length nature of only two have been determined so far. [provided by RefSeq Jul 2008]

Gene Symbol:   CDK11A
Gene Name:   cyclin-dependent kinase 11A
Aliases:   CDC2L2, CDC2L3, CDK11-p110, CDK11-p46, CDK11-p58, MGC131975, PITSLRE, p58GTA
RefSeq:   NC_000001.10 NT_004350.19
Ensembl:   ENSG00000008128
Entrez:   728642
Chromosome Mapping:   1p36.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.995700
y-intercept 37.120000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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