PrimePCR™ Probe Assay: GPM6A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: GPM6A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GPM6A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041343
Assay Design:   exonic
Chromosome Location:   4:176622752-176622863question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000280187 ENST00000393658 ENST00000506894 ENST00000515090 ENST00000503397 ENST00000512610 ENST00000502754 ENST00000507520 ENST00000513667 ENST00000512509 ENST00000505561 ENST00000505375 ENST00000513365 ENST00000509865 ENST00000512897 ENST00000507540 ENST00000505304

Gene Information

Description Not Available

Gene Symbol:   GPM6A
Gene Name:   glycoprotein M6A
Aliases:   GPM6, M6A
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000150625
Entrez:   2823
Chromosome Mapping:   4q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.450000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
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3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
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