PrimePCR™ Probe Assay: MBD1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MBD1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MBD1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041198
Assay Design:   exonic
Chromosome Location:   18:47800030-47800141question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000585672 ENST00000592060 ENST00000382948 ENST00000353909 ENST00000591416 ENST00000339998 ENST00000590208 ENST00000587605 ENST00000588937 ENST00000585595 ENST00000589541 ENST00000398495 ENST00000457839 ENST00000398493 ENST00000398488 ENST00000591535 ENST00000589733 ENST00000269471 ENST00000424334 ENST00000269468 ENST00000347968 ENST00000436910 ENST00000349085

Gene Information

The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq Feb 2011]

Gene Symbol:   MBD1
Gene Name:   methyl-CpG binding domain protein 1
Aliases:   CXXC3, PCM1, RFT
RefSeq:   NC_000018.9 NT_010966.14
Ensembl:   ENSG00000141644
Entrez:   4152
Chromosome Mapping:   18q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.740000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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