PrimePCR™ Probe Assay: TRIM16, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TRIM16, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TRIM16, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041113
Assay Design:   exonic
Chromosome Location:   17:15539350-15539443question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000552983 ENST00000313084 ENST00000455584 ENST00000577886 ENST00000416464 ENST00000578237 ENST00000577446 ENST00000579219 ENST00000580110 ENST00000577372 ENST00000578744 ENST00000336708 ENST00000303575 ENST00000272845 ENST00000333907 ENST00000439458 ENST00000539183 ENST00000572555 ENST00000449552 ENST00000571708 ENST00000573443 ENST00000395671 ENST00000571542 ENST00000414850 ENST00000424146 ENST00000574042 ENST00000395902 ENST00000395672 ENST00000265381 ENST00000354624 ENST00000395086 ENST

Gene Information

This gene was identified as an estrogen and anti-estrogen regulated gene in epithelial cells stably expressing estrogen receptor. The protein encoded by this gene contains two B box domains and a coiled-coiled region that are characteristic of the B box zinc finger protein family. The proteins of this family have been reported to be involved in a variety of biological processes including cell growth differentiation and pathogenesis. Expression of this gene was detected in most tissues. Its function however has not yet been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   TRIM16
Gene Name:   tripartite motif containing 16
Aliases:   DKFZp434O1826, EBBP
RefSeq:   NC_000017.10 NT_010718.16
Ensembl:   ENSG00000221926
Entrez:   10626
Chromosome Mapping:   17p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.650000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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