PrimePCR™ Probe Assay: CYHR1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CYHR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CYHR1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040981
Assay Design:   exonic
Chromosome Location:   8:145690226-145690373question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000311764 ENST00000407673 ENST00000401624 ENST00000404898 ENST00000402142 ENST00000336649 ENST00000400164 ENST00000438911 ENST00000526887 ENST00000403000 ENST00000424149 ENST00000306145 ENST00000533764 ENST00000530637 ENST00000439627 ENST00000360737 ENST00000334179 ENST00000340253 ENST00000339468 ENST00000368764 ENST00000392667 ENST00000361217 ENST00000368646 ENST00000304613 ENST00000368571 ENST00000368572 ENST00000290649

Gene Information

Description Not Available

Gene Symbol:   CYHR1
Gene Name:   cysteine/histidine-rich 1
Aliases:   CHRP, KIAA0496, MGC13010
RefSeq:   NC_000008.10 NT_037704.5
Ensembl:   ENSG00000187954
Entrez:   50626
Chromosome Mapping:   8q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.640000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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