PrimePCR™ Probe Assay: LCN10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LCN10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LCN10, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040980
Assay Design:   exonic
Chromosome Location:   9:139635704-139635805question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000323798 ENST00000263276 ENST00000491143 ENST00000262095 ENST00000356447 ENST00000396671 ENST00000524782 ENST00000426335 ENST00000356798 ENST00000358164 ENST00000174618 ENST00000374314 ENST00000413113 ENST00000497771 ENST00000527229 ENST00000358011 ENST00000442972 ENST00000369738 ENST00000375911 ENST00000306422 ENST00000586421 ENST00000344034 ENST00000350803 ENST00000369902 ENST00000254457 ENST00000418357 ENST00000431256 ENST00000366431 ENST00000521180 ENST00000319914 ENST00000399640 ENST

Gene Information

Members of the lipocalin family such as LCN10 have a common structure consisting of an 8-stranded antiparallel beta-barrel that forms a cup-shaped ligand-binding pocket or calyx. Lipocalins generally bind small hydrophobic ligands and transport them to specific cells (Suzuki et al. 2004 [PubMed 15363845]).[supplied by OMIM Aug 2009]

Gene Symbol:   LCN10
Gene Name:   lipocalin 10
Aliases:   Not Available
RefSeq:   NC_000009.11 NT_024000.16
Ensembl:   ENSG00000187922
Entrez:   414332
Chromosome Mapping:   9q34.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.420000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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