PrimePCR™ Probe Assay: RDM1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RDM1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RDM1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040977
Assay Design:   exonic
Chromosome Location:   17:34252519-34257094question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000431884 ENST00000293273 ENST00000394529 ENST00000419453 ENST00000591402 ENST00000425909 ENST00000430160 ENST00000394528 ENST00000394527

Gene Information

This gene encodes a protein involved in the cellular response to cisplatin a drug commonly used in chemotherapy. The protein encoded by this gene contains two motifs: a motif found in RAD52 a protein that functions in DNA double-strand breaks and homologous recombination and an RNA recognition motif (RRM) that is not found in RAD52. The RAD52 motif region in RAD52 is important for protein function and may be involved in DNA binding or oligomerization. Alternatively spliced transcript variants encoding different isoforms have been reported. [provided by RefSeq Jul 2008]

Gene Symbol:   RDM1
Gene Name:   RAD52 motif 1
Aliases:   MGC33977, RAD52B
RefSeq:   NC_000017.10 NT_010799.15
Ensembl:   ENSG00000187456
Entrez:   201299
Chromosome Mapping:   17q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.750000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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