PrimePCR™ Probe Assay: NDUFA13, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NDUFA13, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NDUFA13, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040966
Assay Design:   exonic
Chromosome Location:   19:19636991-19638101question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000233638 ENST00000373277 ENST00000420366 ENST00000314830 ENST00000373276 ENST00000373274 ENST00000429553 ENST00000507754 ENST00000503283 ENST00000428459 ENST00000252576 ENST00000512771 ENST00000553705 ENST00000555938 ENST00000586674

Gene Information

This gene encodes a subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which functions in the transfer of electrons from NADH to the respiratory chain. The protein is required for complex I assembly and electron transfer activity. The protein binds the signal transducers and activators of transcription 3 (STAT3) transcription factor and can function as a tumor suppressor. The human protein purified from mitochondria migrates at approximately 16 kDa. Transcripts originating from an upstream promoter and capable of expressing a protein with a longer N-terminus have been found but their biological validity has not been determined. [provided by RefSeq Oct 2009]

Gene Symbol:   NDUFA13
Gene Name:   NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13
Aliases:   B16.6, CDA016, FLJ58045, FLJ59191, GRIM-19, GRIM19
RefSeq:   NC_000019.9 NG_013380.1 NT_011295.11
Ensembl:   ENSG00000186010
Entrez:   51079
Chromosome Mapping:   19p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 34.920000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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