PrimePCR™ Probe Assay: GTF2H2C, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: GTF2H2C, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GTF2H2C, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040950
Assay Design:   exonic
Chromosome Location:   5:68862794-68863584question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000575673 ENST00000576299 ENST00000573143 ENST00000574713 ENST00000577126 ENST00000570722 ENST00000512736 ENST00000510979 ENST00000514162 ENST00000380729 ENST00000507595 ENST00000508344 ENST00000274400 ENST00000330280 ENST00000517900 ENST00000425596

Gene Information

Description Not Available

Gene Symbol:   GTF2H2C
Gene Name:   general transcription factor IIH, polypeptide 2C
Aliases:   DKFZp686P18101, FLJ77682, GTF2H2D, MGC102806, MGC198575
RefSeq:   NC_000005.9 NT_006713.15
Ensembl:   ENSG00000183474
Entrez:   728340
Chromosome Mapping:   5q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.620000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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