PrimePCR™ Probe Assay: ARHGAP27, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ARHGAP27, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ARHGAP27, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040823
Assay Design:   exonic
Chromosome Location:   17:43482290-43482403question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000305737 ENST00000394764 ENST00000532038 ENST00000376922 ENST00000528384 ENST00000532891 ENST00000528677 ENST00000428638 ENST00000442348 ENST00000455881 ENST00000548772 ENST00000546802 ENST00000549653

Gene Information

Rho (see ARHA; MIM 165390)-like small GTPases are involved in many cellular processes and they are inactive in the GDP-bound state and active in the GTP-bound state. GTPase-activating proteins such as ARHGAP27 inhibit Rho-like proteins by stimulating their intrinsic GTPase activity (Katoh and Katoh 2004 [PubMed 15492870]).[supplied by OMIM Mar 2008]

Gene Symbol:   ARHGAP27
Gene Name:   Rho GTPase activating protein 27
Aliases:   CAMGAP1, FLJ35572, FLJ43547, FLJ76025, MGC120624, MGC78393, PP905, SH3D20, SH3P20
RefSeq:   NC_000017.10 NT_010783.15 NT_167251.1
Ensembl:   ENSG00000159314
Entrez:   201176
Chromosome Mapping:   17q21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997300
y-intercept 35.380000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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