PrimePCR™ Probe Assay: GJA9, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GJA9, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GJA9, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040755
Assay Design:   exonic
Chromosome Location:   1:39341567-39341658question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000360786 ENST00000454994 ENST00000357771

Gene Information

Connexins such as GJA9 are involved in the formation of gap junctions intercellular conduits that directly connect the cytoplasms of contacting cells. Each gap junction channel is formed by docking of 2 hemichannels each of which contains 6 connexin subunits (Sohl et al. 2003 [PubMed 12881038]).[supplied by OMIM Mar 2008]

Gene Symbol:   GJA9
Gene Name:   gap junction protein, alpha 9, 59kDa
Aliases:   CX58, CX59, GJA10, MGC50985
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000131233
Entrez:   81025
Chromosome Mapping:   1p34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 35.150000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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