PrimePCR™ Probe Assay: CAPN3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CAPN3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CAPN3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040672
Assay Design:   exonic
Chromosome Location:   15:42703108-42703486question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000370355 ENST00000238831 ENST00000357568 ENST00000349748 ENST00000397163 ENST00000397200 ENST00000569827 ENST00000337571 ENST00000569136 ENST00000561817 ENST00000397204 ENST00000564503 ENST00000356316 ENST00000318023 ENST00000236959 ENST00000368820 ENST00000377075

Gene Information

Calpain a heterodimer consisting of a large and a small subunit is a major intracellular protease although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. [provided by RefSeq Jul 2008]

Gene Symbol:   CAPN3
Gene Name:   calpain 3, (p94)
Aliases:   CANP3, CANPL3, LGMD2, LGMD2A, MGC10767, MGC11121, MGC14344, MGC4403, nCL-1, p94
RefSeq:   NC_000015.9 NG_008660.1 NT_010194.17
Ensembl:   ENSG00000092529
Entrez:   825
Chromosome Mapping:   15q15.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.995600
y-intercept 36.450000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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