PrimePCR™ Probe Assay: ZNF302, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ZNF302, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF302, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040669
Assay Design:   exonic
Chromosome Location:   19:35173685-35174068question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000502743 ENST00000506901 ENST00000457781 ENST00000505163 ENST00000505242 ENST00000423823 ENST00000507959 ENST00000446502 ENST00000505365 ENST00000599244 ENST00000392232 ENST00000595708 ENST00000593781 ENST00000492450 ENST00000459757

Gene Information

Description Not Available

Gene Symbol:   ZNF302
Gene Name:   zinc finger protein 302
Aliases:   HSD16, MST154, MSTP154, ZNF135L, ZNF140L, ZNF327
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000089335
Entrez:   55900
Chromosome Mapping:   19q13.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.820000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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