PrimePCR™ Probe Assay: POU2F2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: POU2F2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: POU2F2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040645
Assay Design:   exonic
Chromosome Location:   19:42600234-42600345question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000547525 ENST00000332082 ENST00000549903 ENST00000261400 ENST00000395148 ENST00000373229 ENST00000425054 ENST00000373227 ENST00000520164 ENST00000506455 ENST00000511108 ENST00000511507 ENST00000455639 ENST00000393832 ENST00000296518 ENST00000513574 ENST00000300456 ENST00000342301 ENST00000528894 ENST00000529952 ENST00000560398 ENST00000560558 ENST00000529067 ENST00000526816 ENST00000560804 ENST00000533720 ENST00000389341

Gene Information

The protein encoded by this gene is a homeobox-containing transcription factor of the POU domain family. The encoded protein binds the octamer sequence 5'-ATTTGCAT-3' a common transcription factor binding site in immunoglobulin gene promoters. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2011]

Gene Symbol:   POU2F2
Gene Name:   POU class 2 homeobox 2
Aliases:   OCT2, OTF2, Oct-2
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000028277
Entrez:   5452
Chromosome Mapping:   19q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.590000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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