PrimePCR™ Probe Assay: AGMO, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: AGMO, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AGMO, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040381
Assay Design:   exonic
Chromosome Location:   7:15405139-15405768question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000342526 ENST00000407277 ENST00000418075

Gene Information

Alkylglycerol monooxygenase (AGMO; EC 1.14.16.5) cleaves the O-alkyl bonds of ether lipids which are essential components of brain membranes and function in cell signaling and other critical biologic processes. The reaction catalyzed by AGMO is dependent on tetrahydrobiopterin a metabolite structurally related to the vitamins folic acid and riboflavin (summary by Watschinger et al. 2010 [PubMed 20643956]).[supplied by OMIM Feb 2011]

Gene Symbol:   AGMO
Gene Name:   alkylglycerol monooxygenase
Aliases:   FLJ16237, MGC131748, TMEM195
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000187546
Entrez:   392636
Chromosome Mapping:   7p21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.830000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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