PrimePCR™ Probe Assay: ENKUR, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ENKUR, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ENKUR, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040333
Assay Design:   exonic
Chromosome Location:   10:25279392-25279528question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000331161 ENST00000376363 ENST00000483339

Gene Information

Enkurin interacts with transient receptor potential canonical (TRPC) cation channels (see TRPC1; MIM 602343) and functions as an adaptor protein tethering signal transduction proteins to TRPC channels (Sutton et al. 2004 [PubMed 15385169]).[supplied by OMIM Apr 2009]

Gene Symbol:   ENKUR
Gene Name:   enkurin, TRPC channel interacting protein
Aliases:   C10orf63, DKFZp781F21103, MGC26778, enkurin
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000151023
Entrez:   219670
Chromosome Mapping:   10p12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.910000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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