PrimePCR™ Probe Assay: TRIM34, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TRIM34, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TRIM34, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040279
Assay Design:   exonic
Chromosome Location:   11:5653504-5653638question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000440411 ENST00000257931 ENST00000548043 ENST00000425394 ENST00000260402 ENST00000557821 ENST00000456256 ENST00000591601 ENST00000250896 ENST00000354852 ENST00000514226 ENST00000429814 ENST00000599630 ENST00000338581 ENST00000339598 ENST00000402415 ENST00000272371 ENST00000403946 ENST00000314167 ENST00000511163 ENST00000511980 ENST00000394980 ENST00000508372 ENST00000264790 ENST00000313077 ENST00000560848 ENST00000426216 ENST00000307671 ENST00000543761 ENST00000572705 ENST00000399759 ENST

Gene Information

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains a RING B-box type 1 and B-box type 2 domain and a coiled-coil region. Expression of this gene is up-regulated by interferon. This gene is mapped to chromosome 11p15 where it resides within a TRIM gene cluster. Alternative splicing results in multiple transcript variants. A read-through transcript from the upstream TRIM6 gene has also been observed which results in a fusion product from these neighboring family members. [provided by RefSeq Oct 2010]

Gene Symbol:   TRIM34
Gene Name:   tripartite motif containing 34
Aliases:   IFP1, RNF21
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000258659
Entrez:   53840
Chromosome Mapping:   11p15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.380000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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