PrimePCR™ Probe Assay: PRAMEF4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PRAMEF4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PRAMEF4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040228
Assay Design:   exonic
Chromosome Location:   1:12943006-12943122question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000311413 ENST00000430215 ENST00000233838 ENST00000403733 ENST00000513834 ENST00000448232 ENST00000504005 ENST00000508747 ENST00000357022 ENST00000301295 ENST00000587891 ENST00000226279 ENST00000400237 ENST00000248980 ENST00000248983 ENST00000400236 ENST00000397468 ENST00000249007 ENST00000382088 ENST00000382084 ENST00000400234 ENST00000601806 ENST00000595515 ENST00000598633 ENST00000433867 ENST00000257861 ENST00000263674 ENST00000437584 ENST00000535591 ENST00000235349 ENST00000355096 ENST

Gene Information

Description Not Available

Gene Symbol:   PRAMEF4
Gene Name:   PRAME family member 4
Aliases:   RP5-845O24.6
RefSeq:   NC_000001.10 NT_021937.19
Ensembl:   ENSG00000243073
Entrez:   400735
Chromosome Mapping:   1p36.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.710000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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