PrimePCR™ Probe Assay: CT45A2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CT45A2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CT45A2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040221
Assay Design:   exonic
Chromosome Location:   X:134872096-134872191question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000370741 ENST00000370736 ENST00000495729 ENST00000370734 ENST00000443882 ENST00000434966 ENST00000494421 ENST00000420087 ENST00000370724 ENST00000491480 ENST00000448053 ENST00000472834 ENST00000449726 ENST00000594565 ENST00000596640 ENST00000598716 ENST00000595754 ENST00000594117 ENST00000601753

Gene Information

This gene represents one of a cluster of six similar genes located on the q arm of chromosome X. The genes in this cluster encode members of the cancer/testis (CT) family of antigens and are distinct from other CT antigens. These antigens are thought to be novel therapeutic targets for human cancers. [provided by RefSeq May 2010]

Gene Symbol:   CT45A2
Gene Name:   cancer/testis antigen family 45, member A2
Aliases:   CT45-2, CT45.2
RefSeq:   NC_000023.10 NG_021269.1 NT_011786.16
Ensembl:   ENSG00000242185
Entrez:   728911
Chromosome Mapping:   Xq26.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.310000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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