PrimePCR™ Probe Assay: TSPY10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TSPY10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TSPY10, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040200
Assay Design:   exonic
Chromosome Location:   Y:9368075-9368218question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000320701 ENST00000383042 ENST00000426950 ENST00000383008 ENST00000287721 ENST00000383000 ENST00000383005 ENST00000330628 ENST00000457222 ENST00000424594 ENST00000440483 ENST00000427871 ENST00000451548 ENST00000423647 ENST00000440215 ENST00000446779 ENST00000428845 ENST00000444056 ENST00000429039

Gene Information

Description Not Available

Gene Symbol:   TSPY10
Gene Name:   testis specific protein, Y-linked 10
Aliases:   Not Available
RefSeq:   NC_000024.9 NT_011878.9
Ensembl:   ENSG00000236424
Entrez:   100289087
Chromosome Mapping:   Yp11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.620000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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