PrimePCR™ Probe Assay: PPIAL4G, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PPIAL4G, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PPIAL4G, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040198
Assay Design:   exonic
Chromosome Location:   1:143767228-143767354question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000530673 ENST00000279281 ENST00000261405 ENST00000371790 ENST00000341233 ENST00000361306 ENST00000380602 ENST00000258930 ENST00000539011 ENST00000400794 ENST00000369962 ENST00000361054 ENST00000369965 ENST00000357672 ENST00000268182 ENST00000225983 ENST00000336057 ENST00000586802 ENST00000393622 ENST00000320955 ENST00000253506 ENST00000397790 ENST00000318065 ENST00000329101 ENST00000419275 ENST00000540273 ENST00000304987 ENST00000539781 ENST00000544708 ENST00000369222 ENST00000309680 ENST

Gene Information

Description Not Available

Gene Symbol:   PPIAL4G
Gene Name:   peptidylprolyl isomerase A (cyclophilin A)-like 4G
Aliases:   BX284650.5, FLJ16804, MGC163248, MGC163250, PPIAL4A
RefSeq:   NC_000001.10 NT_113797.1
Ensembl:   ENSG00000236334
Entrez:   644591
Chromosome Mapping:   1q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.920000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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