PrimePCR™ Probe Assay: HLA-DQB2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HLA-DQB2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HLA-DQB2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040183
Assay Design:   exonic
Chromosome Location:   6:32729541-32729684question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000419685 ENST00000455520 ENST00000456529 ENST00000418419 ENST00000549642 ENST00000565184 ENST00000335181 ENST00000565154 ENST00000568459 ENST00000561609 ENST00000564178 ENST00000562997 ENST00000567087 ENST00000319622 ENST00000389093 ENST00000430849 ENST00000417780 ENST00000433801 ENST00000424579 ENST00000547371 ENST00000424530 ENST00000399088 ENST00000399065 ENST00000413089 ENST00000432486 ENST00000428972 ENST00000442043 ENST00000447979 ENST00000548910 ENST00000383121 ENST00000548953 ENST

Gene Information

HLA-DQB2 belongs to the family of HLA class II beta chain paralogs. Class II molecules are heterodimers consisting of an alpha (DQA) and a beta chain (DQB) both anchored in the membrane. They play a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes dendritic cells macrophages). Polymorphisms in the alpha and beta chains specify the peptide binding specificity and typing for these polymorphisms is routinely done for bone marrow transplantation. However this gene HLA-DQB2 is not routinely typed as it is not thought to have an effect on transplantation. There is conflicting evidence in the literature and public sequence databases for the protein-coding capacity of HLA-DQB2. Because there is evidence of transcription and an intact ORF HLA-DQB2 is represented in Entrez Gene and in RefSeq as a protein-coding locus. [provided by RefSeq Oct 2010]

Gene Symbol:   HLA-DQB2
Gene Name:   major histocompatibility complex, class II, DQ beta 2
Aliases:   HLA-DQB1, HLA-DXB
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000232629
Entrez:   3120
Chromosome Mapping:   6p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.980000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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