PrimePCR™ Probe Assay: PGA4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PGA4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PGA4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040168
Assay Design:   exonic
Chromosome Location:   11:60998898-60999038question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000444830 ENST00000383653 ENST00000310638 ENST00000420178 ENST00000511828 ENST00000419873 ENST00000438447 ENST00000282493 ENST00000415976 ENST00000456140 ENST00000450817 ENST00000302754 ENST00000392547 ENST00000272746 ENST00000359761 ENST00000392546 ENST00000409891 ENST00000409415 ENST00000587939 ENST00000415087 ENST00000377173 ENST00000325558 ENST00000543125 ENST00000538258 ENST00000378149 ENST00000537932 ENST00000544899 ENST00000422676 ENST00000312403 ENST00000451616 ENST00000541528 ENST

Gene Information

Description Not Available

Gene Symbol:   PGA4
Gene Name:   pepsinogen 4, group I (pepsinogen A)
Aliases:   FLJ58952, FLJ77962, PGA3, PGA5
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000229183
Entrez:   643847
Chromosome Mapping:   11q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.370000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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