PrimePCR™ Probe Assay: SPANXB2, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040156
Assay Design:   exonic
Chromosome Location:   X:140085682-140085773question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000586582 ENST00000301293 ENST00000549706 ENST00000553030 ENST00000042931 ENST00000330055 ENST00000264042 ENST00000255198 ENST00000264360 ENST00000449283 ENST00000370530 ENST00000358993 ENST00000370519 ENST00000370518 ENST00000370515 ENST00000299320 ENST00000590191 ENST00000219478 ENST00000598204 ENST00000594260 ENST00000597736 ENST00000600641 ENST00000597815

Gene Information

Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family of cancer/testis-associated genes which are located in a cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular gene maps to chromosome X in a head-to-tail orientation with SPANX family member B1 and appears to be a duplication of that locus. The SPANXB genes are unique members of this gene family since they contain an additional 18 nt in their coding region compared to the majority of family members. Although the protein encoded by this gene contains consensus nuclear localization signals the major site for subcellular localization of expressed protein is in the cytoplasmic droplets of ejaculated spermatozoa. This protein provides a biochemical marker for studying the unique structures in spermatazoa while attempting to further define its role in spermatogenesis. [provided by RefSeq Jul 2008]

Gene Symbol:   SPANXB2
Gene Name:   SPANX family, member B2
RefSeq:   NC_000023.10 NT_011786.16
Ensembl:   ENSG00000227234
Entrez:   100133171
UniGene:   Hs.434105
Chromosome Mapping:   Xq27.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.970000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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