PrimePCR™ Probe Assay: GOLGA6L10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: GOLGA6L10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GOLGA6L10, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040102
Assay Design:   exonic
Chromosome Location:   15:82641585-82641676question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000532622 ENST00000526619 ENST00000337838 ENST00000525590 ENST00000329523 ENST00000340249 ENST00000544495 ENST00000333156 ENST00000509311 ENST00000414865 ENST00000448387 ENST00000434739 ENST00000439287 ENST00000300515 ENST00000373631 ENST00000373629 ENST00000559949 ENST00000557886 ENST00000561062 ENST00000358583 ENST00000317716 ENST00000478080 ENST00000466045 ENST00000542731 ENST00000343407 ENST00000450802 ENST00000510439 ENST00000290438 ENST00000576650

Gene Information

Description Not Available

Gene Symbol:   GOLGA6L10
Gene Name:   golgin A6 family-like 10
Aliases:   Not Available
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000205281
Entrez:   647042
Chromosome Mapping:   15q25.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.410000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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