PrimePCR™ Probe Assay: GPANK1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: GPANK1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GPANK1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040045
Assay Design:   exonic
Chromosome Location:   6:31632192-31632341question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000254770 ENST00000505871 ENST00000453908 ENST00000426732 ENST00000312808 ENST00000433880 ENST00000414528 ENST00000457505 ENST00000453899 ENST00000426326 ENST00000424956 ENST00000424195 ENST00000424420 ENST00000418462 ENST00000416364 ENST00000416384 ENST00000418502 ENST00000429266 ENST00000440777 ENST00000422131 ENST00000429097 ENST00000412886 ENST00000415256 ENST00000430230 ENST00000442498 ENST00000443185 ENST00000375906 ENST00000375896 ENST00000375893 ENST00000375895 ENST00000375900 ENST

Gene Information

This gene is located in a cluster of HLA-B-associated transcripts which is included in the human major histocompatability complex III region. This gene encodes a protein which is thought to play a role in immunity. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq Nov 2010]

Gene Symbol:   GPANK1
Gene Name:   G patch domain and ankyrin repeats 1
Aliases:   ANKRD59, BAT4, D6S54E, G5, GPATCH10
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204438
Entrez:   7918
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.560000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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