PrimePCR™ Probe Assay: HSPA1A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HSPA1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HSPA1A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040036
Assay Design:   exonic
Chromosome Location:   6:31784750-31784877question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000300714 ENST00000374769 ENST00000383390 ENST00000400040 ENST00000383389 ENST00000548079 ENST00000391555 ENST00000263666 ENST00000462146 ENST00000466780 ENST00000479530 ENST00000492909 ENST00000535920 ENST00000375654 ENST00000417199 ENST00000375651 ENST00000458062 ENST00000375650 ENST00000545241 ENST00000426095 ENST00000430065 ENST00000422919 ENST00000549607 ENST00000450744 ENST00000417601 ENST00000433487 ENST00000452298 ENST00000550810 ENST00000445736 ENST00000456772 ENST00000547145 ENST

Gene Information

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq Jul 2008]

Gene Symbol:   HSPA1A
Gene Name:   heat shock 70kDa protein 1A
Aliases:   FLJ54303, FLJ54370, FLJ54392, FLJ54408, FLJ75127, HSP70-1, HSP70-1A, HSP70I, HSP72, HSPA1, HSPA1B
RefSeq:   NC_000006.11 NG_011855.1 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167248.1
Ensembl:   ENSG00000204389
Entrez:   3303
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.520000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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