PrimePCR™ Probe Assay: AGPAT1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: AGPAT1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AGPAT1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040024
Assay Design:   exonic
Chromosome Location:   6:32138228-32138367question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000430777 ENST00000440840 ENST00000439774 ENST00000424030 ENST00000416363 ENST00000433896 ENST00000434614 ENST00000452427 ENST00000430226 ENST00000425572 ENST00000436149 ENST00000448196 ENST00000371158 ENST00000457923 ENST00000446463 ENST00000449776 ENST00000427763 ENST00000446323 ENST00000425204 ENST00000242057 ENST00000399827 ENST00000399833 ENST00000399825 ENST00000399830 ENST00000399829 ENST00000383294 ENST00000267119 ENST00000414520 ENST00000414933 ENST00000444369 ENST00000456421 ENST

Gene Information

This gene encodes an enzyme that converts lysophosphatidic acid (LPA) into phosphatidic acid (PA). LPA and PA are two phospholipids involved in signal transduction and in lipid biosynthesis in cells. This enzyme localizes to the endoplasmic reticulum. This gene is located in the class III region of the human major histocompatibility complex. Alternative splicing results in two transcript variants encoding the same protein. [provided by RefSeq Jul 2008]

Gene Symbol:   AGPAT1
Gene Name:   1-acylglycerol-3-phosphate O-acyltransferase 1 (lysophosphatidic acid acyltransferase, alpha)
Aliases:   1-AGPAT1, G15, LPAAT-alpha, LPAATA, MGC4007, MGC5423
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204310
Entrez:   10554
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.110000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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