This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: DAXX, Human
PrimePCR™ Template for Probe Assay: DAXX, Human
This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins such as apoptosis antigen Fas centromere protein C and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications including sumoylation phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq Nov 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.