PrimePCR™ Probe Assay: HLA-DQA1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HLA-DQA1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HLA-DQA1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039978
Assay Design:   exonic
Chromosome Location:   6:32609800-32609942question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000393930 ENST00000310706 ENST00000393931 ENST00000389054 ENST00000520569 ENST00000518047 ENST00000398562 ENST00000389057 ENST00000398566 ENST00000398557 ENST00000253811 ENST00000449560 ENST00000546801 ENST00000402050 ENST00000438376 ENST00000325979 ENST00000404984 ENST00000480290 ENST00000435512 ENST00000453504 ENST00000457246 ENST00000442315 ENST00000450960 ENST00000447735 ENST00000551533 ENST00000456258 ENST00000450614 ENST00000441297 ENST00000420385 ENST00000241802 ENST00000274181 ENST

Gene Information

HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB) both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes dendritic cells macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide exons 2 and 3 encode the two extracellular domains and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. [provided by RefSeq Jul 2008]

Gene Symbol:   HLA-DQA1
Gene Name:   major histocompatibility complex, class II, DQ alpha 1
Aliases:   CD, CELIAC1, DQ-A1, FLJ27088, FLJ27328, GSE, HLA-DQA, MGC149527
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000196735
Entrez:   3117
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.990000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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