PrimePCR™ Probe Assay: AC131180.1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: AC131180.1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AC131180.1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039958
Assay Design:   exonic
Chromosome Location:   2:132022154-132022243question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000575087 ENST00000575842 ENST00000573283 ENST00000331925 ENST00000591446 ENST00000300952 ENST00000317568 ENST00000355991 ENST00000416776 ENST00000252590 ENST00000290378 ENST00000361387 ENST00000409731 ENST00000345517 ENST00000409624 ENST00000419548 ENST00000344754 ENST00000202578 ENST00000409832 ENST00000551509 ENST00000359373 ENST00000334211 ENST00000393609 ENST00000426523 ENST00000429686 ENST00000455638 ENST00000331789 ENST00000171887 ENST00000481893 ENST00000541884 ENST00000312513 ENST

Gene Information

Description Not Available

Gene Symbol:   AC131180.1
Gene Name:   POTE ankyrin domain family member E
Aliases:   A26C1, A26C1A, CT104.2, POTE-2, POTE2, POTE2gamma
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000188219
Entrez:   445582
Chromosome Mapping:   2q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.240000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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