PrimePCR™ Probe Assay: H2AFB3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: H2AFB3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: H2AFB3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039939
Assay Design:   exonic
Chromosome Location:   X:154689208-154689332question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000289409 ENST00000358522 ENST00000545385 ENST00000394770 ENST00000254730 ENST00000483457 ENST00000474275 ENST00000354461 ENST00000489426 ENST00000354514 ENST00000369444 ENST00000280886 ENST00000381496 ENST00000301012 ENST00000329117 ENST00000361588 ENST00000595821 ENST00000594123 ENST00000599985 ENST00000596716

Gene Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A H2B H3 and H4). The chromatin fiber is further compacted through the interaction of a linker histone H1 with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. This gene is part of a region that is repeated three times on chromosome X once in intron 22 of the F8 gene and twice closer to the Xq telomere. This record represents the most telomeric copy. [provided by RefSeq Jul 2008]

Gene Symbol:   H2AFB3
Gene Name:   H2A histone family, member B3
Aliases:   H2ABBD, H2AFB, H2AFB2
RefSeq:   NC_000023.10 NG_005114.1 NT_167198.1
Ensembl:   ENSG00000185978
Entrez:   83740
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.470000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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