PrimePCR™ Probe Assay: RP11-82O18.1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RP11-82O18.1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RP11-82O18.1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039919
Assay Design:   exonic
Chromosome Location:   16:15460510-15463575question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000399147 ENST00000547494 ENST00000542601 ENST00000222271 ENST00000425807 ENST00000526520 ENST00000531598 ENST00000552140 ENST00000530328 ENST00000529166 ENST00000553201 ENST00000328085 ENST00000424549 ENST00000537062 ENST00000265678 ENST00000366863 ENST00000534799 ENST00000547107 ENST00000358815 ENST00000360151 ENST00000534094 ENST00000543801 ENST00000432570 ENST00000404039 ENST00000377770 ENST00000332007 ENST00000427557 ENST00000381497 ENST00000524823 ENST00000527703 ENST00000331436 ENST

Gene Information

Description Not Available

Gene Symbol:   RP11-82O18.1
Gene Name:   Uncharacterized protein
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000183793
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.340000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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