PrimePCR™ Probe Assay: C17orf49, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
Your Price:   Log In
Quantity:  
 

PrimePCR™ PreAmp for Probe Assay: C17orf49, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: C17orf49, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039842
Assay Design:   exonic
Chromosome Location:   17:6920620-6920709question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000536824 ENST00000428720 ENST00000519106 ENST00000407373 ENST00000332585 ENST00000401475 ENST00000431368 ENST00000452656 ENST00000403222 ENST00000382310 ENST00000437268 ENST00000535268 ENST00000217305 ENST00000539905 ENST00000336078 ENST00000591083 ENST00000589288 ENST00000591230 ENST00000591808 ENST00000454914 ENST00000427869 ENST00000396593 ENST00000427052 ENST00000342160 ENST00000262854 ENST00000497194 ENST00000404792 ENST00000378585 ENST00000329152 ENST00000360342 ENST00000337576 ENST

Gene Information

Description Not Available

Gene Symbol:   C17orf49
Gene Name:   chromosome 17 open reading frame 49
Aliases:   BAP18, MGC49942
RefSeq:   NC_000017.10 NT_010718.16
Ensembl:   ENSG00000161939
Entrez:   124944
Chromosome Mapping:   17p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996800
y-intercept 36.600000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download