PrimePCR™ Probe Assay: SIGMAR1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SIGMAR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SIGMAR1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039347
Assay Design:   exonic
Chromosome Location:   9:34635667-34635814question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000378892 ENST00000277010 ENST00000477726

Gene Information

This gene encodes a receptor protein that interacts with a variety of psychotomimetic drugs including cocaine and amphetamines. The receptor is believed to play an important role in the cellular functions of various tissues associated with the endocrine immune and nervous systems. As indicated by its previous name opioid receptor sigma 1 (OPRS1) the product of this gene was erroneously thought to function as an opioid receptor; it is now thought to be a non-opioid receptor. Alternative splicing of this gene results in transcript variants encoding distinct isoforms. [provided by RefSeq Dec 2008]

Gene Symbol:   SIGMAR1
Gene Name:   sigma non-opioid intracellular receptor 1
Aliases:   FLJ25585, MGC3851, OPRS1, SR-BP1, SRBP
RefSeq:   NC_000009.11 NT_008413.18
Ensembl:   ENSG00000147955
Entrez:   10280
Chromosome Mapping:   9p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.110000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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