PrimePCR™ Probe Assay: DMTF1, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best coverage

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039279
Assay Design:   exonic
Chromosome Location:   7:86792811-86792907question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000331242 ENST00000413276 ENST00000446796 ENST00000420131 ENST00000414630 ENST00000453049 ENST00000428819 ENST00000448598 ENST00000449088 ENST00000432937 ENST00000432366 ENST00000423590 ENST00000394703 ENST00000579592 ENST00000434534 ENST00000582925 ENST00000425705 ENST00000394702 ENST00000411766 ENST00000414194

Gene Information

This gene encodes a transcription factor that contains a cyclin D-binding domain three central Myb-like repeats and two flanking acidic transactivation domains at the N- and C-termini. The encoded protein is induced by the oncogenic Ras signaling pathway and functions as a tumor suppressor by activating the transcription of ARF and thus the ARF-p53 pathway to arrest cell growth or induce apoptosis. It also activates the transcription of aminopeptidase N and may play a role in hematopoietic cell differentiation. The transcriptional activity of this protein is regulated by binding of D-cyclins. This gene is hemizygously deleted in approximately 40% of human non-small-cell lung cancer and is a potential prognostic and gene-therapy target for non-small-cell lung cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Dec 2008]

Gene Symbol:   DMTF1
Gene Name:   cyclin D binding myb-like transcription factor 1
Aliases:   DMP1, DMTF, FLJ25188, FLJ41265, FLJ76054, hDMP1
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000135164
Entrez:   9988
UniGene:   Hs.196129
Chromosome Mapping:   7q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.990000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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