PrimePCR™ Probe Assay: CTCFL, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CTCFL, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CTCFL, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039210
Assay Design:   exonic
Chromosome Location:   20:56093856-56094280question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000243914 ENST00000371196 ENST00000423479 ENST00000429804 ENST00000433949 ENST00000502686 ENST00000422109 ENST00000432255 ENST00000539382 ENST00000422869 ENST00000426658

Gene Information

CCCTC-binding factor (CTCF) an 11-zinc-finger factor involved in gene regulation utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. [provided by RefSeq Jul 2008]

Gene Symbol:   CTCFL
Gene Name:   CCCTC-binding factor (zinc finger protein)-like
Aliases:   BORIS, CT27, CTCF-T, HMGB1L1, MGC163358, MGC169105, MGC169106, dJ579F20.2
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000124092
Entrez:   140690
Chromosome Mapping:   20q13.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.690000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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