PrimePCR™ Probe Assay: PLAGL1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PLAGL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PLAGL1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039179
Assay Design:   exonic
Chromosome Location:   6:144263432-144263530question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000360537 ENST00000354765 ENST00000367572 ENST00000367571 ENST00000417959 ENST00000444202 ENST00000429150 ENST00000392309 ENST00000416623 ENST00000437412 ENST00000392307

Gene Information

This gene encodes a C2H2 zinc finger protein with transactivation and DNA-binding activities. It has been shown to have anti-proliferative properties and thus thought to function as a tumor suppressor. In addition overexpression of this gene during fetal development is believed to underlie the rare disorder transient neonatal diabetes mellitus (TNDM). This gene is imprinted with preferential expression of the paternal allele in many tissues however biallelic expression has been noted in peripheral blood leucocytes. A recent study reports that tissue-specific imprinting results from variable utilization of monoallelic and biallelic promoters. Many transcript variants differing in the 5' UTR and encoding two different isoforms have been found for this gene. [provided by RefSeq Oct 2010]

Gene Symbol:   PLAGL1
Gene Name:   pleiomorphic adenoma gene-like 1
Aliases:   DKFZp781P1017, LOT1, MGC126275, MGC126276, ZAC, ZAC1
RefSeq:   NG_016174.1 NC_000006.11 NG_009384.1 NT_025741.15
Ensembl:   ENSG00000118495
Entrez:   5325
Chromosome Mapping:   6q24-q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.760000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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