PrimePCR™ Probe Assay: POLR2H, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: POLR2H, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: POLR2H, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0035881
Assay Design:   Exonic
Chromosome Location:   3:184086223-184086327question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000456318 ENST00000438240 ENST00000430783 ENST00000443489 ENST00000452961 ENST00000429568 ENST00000296223

Gene Information

This gene encodes one of the essential subunits of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases I and III. [provided by RefSeq Jul 2008]

Gene Symbol:   POLR2H
Gene Name:   polymerase (RNA) II (DNA directed) polypeptide H
Aliases:   RPABC3, RPB17, RPB8, hsRPB8
RefSeq:   NC_000003.11 NG_016422.1 NT_005612.16
Ensembl:   ENSG00000163882
Entrez:   5437
Chromosome Mapping:   3q28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 37.030000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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