PrimePCR™ Probe Assay: RPL27A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RPL27A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RPL27A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0033889
Assay Design:   Exonic
Chromosome Location:   11:8707248-8707386question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000314138 ENST00000524496 ENST00000532359 ENST00000530022 ENST00000526562 ENST00000525981 ENST00000530913

Gene Information

Ribosomes the organelles that catalyze protein synthesis consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins multiple processed pseudogenes derived from this gene are dispersed through the genome. [provided by RefSeq Jul 2008]

Gene Symbol:   RPL27A
Gene Name:   ribosomal protein L27a
Aliases:   FLJ43464, L27A, MGC10850, MGC17878, MGC87238
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000166441
Entrez:   6157
Chromosome Mapping:   11p15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.610000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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