PrimePCR™ Probe Assay: TCEB3B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TCEB3B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TCEB3B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0032449
Assay Design:   Exonic
Chromosome Location:   18:44559665-44559788question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000332567

Gene Information

This gene encodes the transcriptionally active subunit of the SIII (or elongin) transcription elongation factor complex which also includes two regulatory subunits elongins B and C. This complex acts to increase the rate of RNA chain elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites along the DNA template. Whereas a related protein with similar function elongin A is ubiquitously expressed the encoded protein is specifically expressed in the testis suggesting it may have a role in spermatogenesis. [provided by RefSeq Jul 2008]

Gene Symbol:   TCEB3B
Gene Name:   transcription elongation factor B polypeptide 3B (elongin A2)
Aliases:   ELOA2, HsT832, MGC119351, TCEB3L
RefSeq:   NC_000018.9 NG_016749.1 NT_010966.14
Ensembl:   ENSG00000206181
Entrez:   51224
Chromosome Mapping:   18q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.480000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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