PrimePCR™ Probe Assay: CEBPB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0032155
Assay Design:   Exonic
Chromosome Location:   20:48808922-48809068question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000303004

Gene Information

The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha CEBP-delta and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene as well as to regulatory regions of several acute-phase and cytokine genes. In addition the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene. [provided by RefSeq Jul 2008]

Gene Symbol:   CEBPB
Gene Name:   CCAAT/enhancer binding protein (C/EBP), beta
Aliases:   C/EBP-beta, CRP2, IL6DBP, LAP, MGC32080, NF-IL6, TCF5
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000172216
Entrez:   1051
Chromosome Mapping:   20q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.190000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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