PrimePCR™ Probe Assay: D2HGDH, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: D2HGDH, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: D2HGDH, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025327
Assay Design:   Exonic
Chromosome Location:   2:242683102-242683228question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000342518 ENST00000537090 ENST00000321264 ENST00000403782 ENST00000417686

Gene Information

This gene encodes D-2hydroxyglutarate dehydrogenase a mitochondrial enzyme belonging to the FAD-binding oxidoreductase/transferase type 4 family. This enzyme which is most active in liver and kidney but also active in heart and brain converts D-2-hydroxyglutarate to 2-ketoglutarate. Mutations in this gene are present in D-2-hydroxyglutaric aciduria a rare recessive neurometabolic disorder causing developmental delay epilepsy hypotonia and dysmorphic features. [provided by RefSeq Jul 2008]

Gene Symbol:   D2HGDH
Gene Name:   D-2-hydroxyglutarate dehydrogenase
Aliases:   D2HGD, FLJ42195, MGC25181
RefSeq:   NC_000002.11 NG_012012.1 NT_005416.13
Ensembl:   ENSG00000180902
Entrez:   728294
Chromosome Mapping:   2q37.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.850000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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