PrimePCR™ Probe Assay: RAMP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RAMP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RAMP1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025211
Assay Design:   Exonic
Chromosome Location:   2:238820378-238820577question
Amplicon Length:   170
Splice Variants Targeted:   ENST00000404910 ENST00000254661 ENST00000409726 ENST00000403885

Gene Information

The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR a receptor with seven transmembrane domains can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor depending on which members of the RAMP family are expressed. In the presence of this (RAMP1) protein CRLR functions as a CGRP receptor. The RAMP1 protein is involved in the terminal glycosylation maturation and presentation of the CGRP receptor to the cell surface. [provided by RefSeq Jul 2008]

Gene Symbol:   RAMP1
Gene Name:   receptor (G protein-coupled) activity modifying protein 1
Aliases:   Not Available
RefSeq:   NC_000002.11 NT_005120.16
Ensembl:   ENSG00000132329
Entrez:   10267
Chromosome Mapping:   2q36-q37.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 34.280000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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