PrimePCR™ Probe Assay: SLC16A10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $208.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024905
Assay Design:   Exonic
Chromosome Location:   6:111498415-111498520question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000368851 ENST00000419619 ENST00000439288 ENST00000368850 ENST00000535637 ENST00000368853

Gene Information

SLC16A10 is a member of a family of plasma membrane amino acid transporters that mediate the Na(+)-independent transport of aromatic amino acids across the plasma membrane.[supplied by OMIM Apr 2004]

Gene Symbol:   SLC16A10
Gene Name:   solute carrier family 16, member 10 (aromatic amino acid transporter)
Aliases:   PRO0813, TAT1
RefSeq:   NC_000006.11 NT_025741.15
Ensembl:   ENSG00000112394
Entrez:   117247
UniGene:   Hs.591327
Chromosome Mapping:   6q21-q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.620000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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