PrimePCR™ Probe Assay: BTN3A2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: BTN3A2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BTN3A2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024851
Assay Design:   Exonic
Chromosome Location:   6:26373151-26373258question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000527422 ENST00000356386 ENST00000377708 ENST00000396948 ENST00000396934 ENST00000535620 ENST00000508906

Gene Information

This gene encodes a member of the butyrophilin (BTN) family and the immunoglobulin (IG) superfamily. The gene resides in the juxta-telomeric region of the major histocompatability class 1 locus and is clustered with the other six family members including BTN BTN2A1 BTN2A2 BTN2A3 BTN3A1 and BTN3A3 on chromosome 6. The full-length protein encoded by this gene is a type I membrane protein. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2010]

Gene Symbol:   BTN3A2
Gene Name:   butyrophilin, subfamily 3, member A2
Aliases:   BT3.2, BT3.3, BTF4, FLJ40011
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000186470
Entrez:   11118
Chromosome Mapping:   6p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.520000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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