PrimePCR™ Probe Assay: TOPORS, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TOPORS, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TOPORS, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024530
Assay Design:   Exonic
Chromosome Location:   9:32543156-32543286question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000360538 ENST00000379858

Gene Information

This gene encodes a nuclear protein which is serine and arginine rich and contains a RING-type zinc finger domain. It is highly expressed in the testis and functions as an ubiquitin-protein E3 ligase. Mutations in this gene are associated with retinitis pigmentosa type 31. Alternatively spliced transcript variants encoding different isoforms have been observed for this locus. [provided by RefSeq Sep 2010]

Gene Symbol:   TOPORS
Gene Name:   topoisomerase I binding, arginine/serine-rich, E3 ubiquitin protein ligase
Aliases:   LUN, P53BP3, RP31, TP53BPL
RefSeq:   NG_011511.1 NC_000009.11 NG_017050.1 NT_008413.18
Ensembl:   ENSG00000197579
Entrez:   10210
Chromosome Mapping:   9p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.480000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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