PrimePCR™ Probe Assay: SH3BP4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SH3BP4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SH3BP4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024377
Assay Design:   Exonic
Chromosome Location:   2:235949559-235949674question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000392011 ENST00000416021 ENST00000409212 ENST00000344528 ENST00000446904 ENST00000420127

Gene Information

This gene encodes a protein with 3 Asn-Pro-Phe (NPF) motifs an SH3 domain a PXXP motif a bipartite nuclear targeting signal and a tyrosine phosphorylation site. This protein is involved in cargo-specific control of clathrin-mediated endocytosis specifically controlling the internalization of a specific protein receptor. [provided by RefSeq Jul 2008]

Gene Symbol:   SH3BP4
Gene Name:   SH3-domain binding protein 4
Aliases:   BOG25, TTP
RefSeq:   NC_000002.11 NT_005120.16
Ensembl:   ENSG00000130147
Entrez:   23677
Chromosome Mapping:   2q37.1-q37.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.780000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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